Interaction of <i>Acanthamoeba</i> T5 with a Vero Cell Culture: An Exploratory Study Using Live-Cell Imaging and Confocal Microscopy.

ABSTRACT: Acanthamoeba is a free-living amoeba widely distributed in nature, responsible for clinical cases of encephalitis and keratitis in humans. Due to the increase in the number of cases in recent years, understanding the damage mechanisms employed by the amoeba is very important for the clinical management of the disease, development of diagnostic tools and identification of therapeutic targets. To date, most experimental studies to determine the virulence factors and pathogenesis of Acanthamoeba have employed genotype T4 as an infection model, resulting in minimal information regarding other genotypes. In this work, we explored the direct and indirect effect of A. lenticulata genotype T5 trophozoites and their excretion/secretion products over a Vero cell monolayer. Using

SUBMITTER: Abrahams-Sandi E 

PROVIDER: S-EPMC12298998 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature