An Evaluation of the Safety, Immunogenicity, and Protective Efficacy of a Combined Diphtheria-Tetanus-Acellular Pertussis, <i>Haemophilus influenzae</i> Type b, and ACYW135 Meningococcal Conjugate Vaccine in Murine and Rat Models.

ABSTRACT: Background: The combined diphtheria-tetanus-acellular pertussis (three-component), Haemophilus influenzae type b (Hib, conjugate), and ACYW135 meningococcal (conjugate) vaccine (DTaP-Hib-MCV4) offers a promising alternative to single-component vaccines, potentially simplifying immunization schedules and improving vaccination coverage. Methods: We evaluated the safety, immunogenicity, and protective efficacy of DTaP-Hib-MCV4 in animal models. Acute and long-term toxicity studies were conducted in Sprague-Dawley (SD) rats with equal numbers of male and female animals. Immunogenicity was assessed in female NIH mice and SD rats using a three-dose regimen at 14-day intervals. Orbital blood was collected 14 days post-immunization to measure IgG titers against pertussis, diph

SUBMITTER: Sui X 

PROVIDER: S-EPMC12299065 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature