Glucoselipid Biosurfactant Biosynthesis Operon of <i>Rouxiella badensis</i> DSM 100043^T: Screening, Identification, and Heterologous Expression in <i>Escherichia coli</i>.

ABSTRACT: Rouxiella badensis DSM 100043^T had been previously proven to produce a novel glucoselipid biosurfactant which has a very low critical micelle concentration (CMC) as well as very good stability against a wide range of pH, temperature, and salinity. In this study, we performed a function-based library screening from a R. badensis DSM 100043^T genome library to identify responsible genes for biosynthesis of this glucoselipid. The identified open reading frames (ORFs) were cloned into several constructs in Escherichia coli for gene permutation analysis and the individual products were analyzed using high-performance thin-layer chromatography (HPTLC). Products of interest from positive expression strains were purified and analyzed by liquid chromatograp

SUBMITTER: Harahap AFP 

PROVIDER: S-EPMC12299268 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature