Comprehensive RNA-Seq Analysis of Human Osteoclast Function in Response to <i>Bothrops moojeni</i> Venom Fractions: Pathways of Bone Resorption and Cytoskeletal Disruption.

ABSTRACT: This study investigated the effects of Bothrops moojeni (B. moojeni) venom and its high- (HMM) and low-molecular mass (LMM) fractions on human osteoclast (OC) differentiation and function in vitro, aiming to identify novel therapeutics for bone disorders. Venom preparations were applied at 5 µg/mL (crude venom and HMM) or 1 µg/mL (LMM) from day 4 of peripheral blood mononuclear cell (PBMC) differentiation through terminal OC formation, enabling evaluation across early differentiation, fusion, and maturation stages. RNA sequencing revealed 7793 genes common to all experimental groups, with unique gene expression signatures of 149 (control), 221 (HMM), 248 (crude venom), and 60 (LMM) genes, reflecting distinct molecular responses. The negative control PBMC group exhibited 1013

SUBMITTER: D'Amelio F 

PROVIDER: S-EPMC12299344 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature