Low Tenacity of <i>Toxoplasma gondii</i> Tachyzoites In Vitro.

ABSTRACT: Toxoplasma gondii, an obligate intracellular protozoan, poses significant risks to public health due to its widespread distribution and potential for severe congenital and neurological complications. The fast-replicating tachyzoite stage is crucial for acute infection and laboratory studies, yet effective inactivation methods remain inadequately explored. This study evaluates various chemical and physical approaches to inactivate T. gondii tachyzoites in vitro. Using a combination of GFP fluorescence and viability assays, we demonstrated the complete inactivation of tachyzoites with ethanol (≥30%), hydrogen peroxide (≥3%), o-hydroxydiphenyl fatty acid eutectic with peracetic acid (≥1%), and heat treatment at 60 °C for 30 min. Our findings highlight the importance of concentra

SUBMITTER: Grochow T 

PROVIDER: S-EPMC12299489 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature