Sequential Extraction of Bioactive Saponins from <i>Cucumaria frondosa</i> Viscera: Supercritical CO₂-Ethanol Synergy for Enhanced Yields and Antioxidant Performance.

ABSTRACT: This study investigates the sequential extraction of lipids and saponins from C. frondosa viscera. Lipids were extracted using supercritical carbon dioxide (scCO₂) in the presence of ethanol (EtOH) as a co-solvent. Subsequently, the lipid-extracted viscera underwent three saponin extraction approaches, scCO₂-scCO₂, scCO₂-EtOH, and scCO₂-hot water, resulting in saponin-rich extracts. Process parameter investigation for saponin extraction from scCO₂-defatted viscera revealed minimal effects of temperature, pressure, extraction time, static extraction, and EtOH concentration on saponin yields, allowing for milder operational conditions (35 °C, 20 MPa, 30 min dynamic extraction, 75% EtOH at 0.5 mL/min) to achieve energy-e

SUBMITTER: Lin J 

PROVIDER: S-EPMC12299899 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature