Human Immune System Reconstitution in NOD/Shi-<i>Prkdc^scid Il2rg^em1</i>/Cyagen Mice to Study HIV Infection: Challenges and Pitfalls.

ABSTRACT: The main challenge after engraftment of human tissues to mice is the development of graft-versus-host disease. It often occurs in an acute form, which reduces the time frame for observations. This is especially important to take into account when planning long-term studies of chronic diseases such as HIV infection. In addition, in mice, even with a similar genotype but different origin, the interaction between the graft and the recipient's organism can manifest itself differently. We engrafted human immune cells in three different concentrations into immunodeficient NOD/Shi-Prkdc^scid Il2rg^em1/Cyagen mice. Then, the initial points of development of a severe graft-versus-host reaction and the maximum possible time window for humane observation were determined. Th

SUBMITTER: Nagornykh AM 

PROVIDER: S-EPMC12300024 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature