Therapeutic Optimization of <i>Pseudomonas aeruginosa</i> Phages: From Isolation to Directed Evolution.

ABSTRACT: Pseudomonas aeruginosa is a major opportunistic pathogen with high levels of antibiotic resistance. Phage therapy represents a promising alternative for the treatment of difficult infections both alone and in combination with antibiotics. Here, we isolated and characterized three novel lytic myoviruses, Cisa, Nello, and Moonstruck. Genomic analysis revealed that Cisa and Nello belong to the Pbunavirus genus, while Moonstruck is a novel Pakpunavirus species. All lacked lysogeny, virulence, or resistance-associated genes, supporting their therapeutic suitability. Phage Nello and Moonstruck were active against P. aeruginosa Pa3GrPv, isolated from a patient with lung infection candidate for phage therapy. Moonstruck exhibited superior lytic activity with ciprofloxac

SUBMITTER: Bolognini S 

PROVIDER: S-EPMC12300175 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature