Cellulolytic Potential of Newly Isolated Alcohol-Tolerant <i>Bacillus methylotrophicus</i>.

ABSTRACT: Reprocessing lignocellulosic waste to obtain new products for industrial purposes is a vital part of circular economy. This paper reports the cellulase production by newly isolated Bacillus methylotrophicus cultured on lignocellulosic agro-industrial by-products, out of which brewer's spent grain (BSG) was selected as most beneficial. Plackett-Burman design was used for screening medium components, while Box-Behnken design was further applied to model the impact of the three most influential variables. The maximum approximated cellulase activity was 0.469 U/mL (1 U = 1 µmol of reducing sugars/1 min), at 48.6 g/L substrate, 5.3 g/L ammonium sulfate, pH 6.1. The partially purified cellulase was characterized, which demonstrated broad range of optimal pH (6.5-9.4), temperature (50-60 °

SUBMITTER: Choinska-Pulit A 

PROVIDER: S-EPMC12300512 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature