Adaptive Evolution of <i>Sporosarcina pasteurii</i> Enhances Saline-Alkali Resistance for High-Performance Concrete Crack Repair via MICP.

ABSTRACT: Microbially induced calcium carbonate precipitation (MICP) has emerged as a research focus in concrete crack remediation due to its environmental compatibility and efficient mineralization capacity. The hypersaline conditions of seawater (average 35 g/L NaCl) and alkaline environments (pH 12) within concrete cracks pose significant challenges to the survival of mineralization-capable microorganisms. To enhance microbial tolerance under these extreme conditions, this study employed a laboratory adaptive evolution strategy to successfully develop a Sporosarcina pasteurii strain demonstrating tolerance to 35 g/L NaCl and pH 12. Comparative analysis of growth characteristics (OD₆₀₀), pH variation, urease activity, and specific urease activity revealed that the evolved strain

SUBMITTER: Liu J 

PROVIDER: S-EPMC12300605 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature