The Dissociation of Latrophilin Fragments by Perfluorooctanoic Acid (PFOA) Inhibits LTX^N4C-Induced Neurotransmitter Release.

ABSTRACT: α-Latrotoxin stimulates neurotransmitter release by binding to a presynaptic receptor and then forming ion-permeable membrane pores and/or stimulating the receptor, latrophilin-1, or Adhesion G-protein-coupled receptor type L1 (ADGRL1). To avoid pore formation, we use the mutant α-latrotoxin (LTX^N4C), which does not form pores and only acts through ADGRL1. ADGRL1 is cleaved into an N-terminal fragment (NTF) and a C-terminal fragment (CTF), which behave as independent cell-surface proteins, reassociating upon binding LTX^N4C. We investigated the role of the NTF-CTF association in LTX^N4C action, using perfluorooctanoic acid (PFOA). We demonstrate that at low concentrations (≤100 μM) PFOA does not adversely affect ADGRL1-expressing neuroblastoma cells or inhibi

SUBMITTER: Petitto E 

PROVIDER: S-EPMC12300799 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature