Analysis of Processing Impact on Raspberries Based on Broad-Spectrum Metabolomics.

ABSTRACT: Objective: Our objective was to explore the regulatory mechanism of salt processing on the metabolome of the raspberry and its potential efficacy against diabetic nephropathy (DN), providing metabolomic and network pharmacological evidence for the scientific connotation of traditional Chinese medicine processing. Methods: Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based metabolomics was used to compare the metabolic profiles between raw and salt-processed raspberries. Network pharmacology was applied to screen the common targets of the active components in the salt-processed raspberry and DN-related pathways, followed by in vitro cell experiments to validate the regulation of the MAPK signaling pathway. Results: The metabolomic

SUBMITTER: Wang X 

PROVIDER: S-EPMC12301035 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature