Transcriptomic profiling of scleroderma monocytes reveals links with cardiovascular complications, implicating Notch and interferon pathways.

ABSTRACT:

Objectives

Recent research has highlighted the critical role of monocytes and macrophages in driving both inflammatory and fibrotic processes in systemic sclerosis. This study seeks to elucidate the gene expression profiles of systemic sclerosis monocytes and their potential links to disease complications, with the ultimate goal of uncovering novel therapeutic targets.

Methods

A total of 48 systemic sclerosis patients and 15 controls were recruited and monocytes were isolated using CD14+ magnetic beads. Total RNA was extracted and bulk RNA-seq analysis was performed. Differential gene expression followed by unsupervised hierarchical clustering and pathway analysis was conducted, and correlations with clinical features were analyzed. Interferon signature score (IFN6) was calc

SUBMITTER: Dinc MT 

PROVIDER: S-EPMC12301231 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature