Feasibility of amino acid profiling in long-term stored formalin-fixed paraffin-embedded colorectal neoplasia tissue.

ABSTRACT:

Introduction

Metabolic processes play a role in cancer development, with faecal amino acids emerging as potential biomarkers for colorectal neoplasia. While fresh frozen tissue is preferred for metabolomic analysis, formalin-fixed paraffin-embedded (FFPE) tissue is more widely available.

Objectives

We aimed to evaluate amino acid profiles in FFPE tissue across different stages of the adenoma-carcinoma sequence.

Method

A panel of 20 amino acids was measured using liquid chromatography-tandem mass spectrometry.

Results

Fourteen amino acids were detected, with proline elevated in colorectal carcinoma versus advanced (FC 2.33, p = 0.04) and non-advanced adenomas (FC 2.42, p = 0.02).

Conclusion

Despite analytical challenges, amino acid profiling in FFPE tissu

SUBMITTER: Opperman RCM 

PROVIDER: S-EPMC12301268 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature