Potential induction of the relative mRNA expression levels of CYP450 by Zhicaowu-Hezi (Aconiti kusnezoffii radix preparata and <i>Terminalia chebula</i> Retz.).

ABSTRACT:

Background

Processed Aconiti Kusnezoffii Radix (Aconiti kusnezoffii Radix Preparata, Zhicaowu) and Terminalia chebula Retz. (Hezi) are a classic herb pair in Mongolian medicine, where Hezi mitigates Zhicaowu's hepatotoxicity. Despite extensive studies on their detoxification effects, the role of cytochrome P450 (CYP450) modulation remains unclear.

Aim

This study aimed to systematically evaluate the regulatory effects of Zhicaowu and Hezi combination on both enzymatic activity and mRNA expression of CYP450 isoforms (CYP1a2, CYP2b1, CYP2c11, CYP2c13, CYP2d2, CYP2e1, and CYP3a1), and to explore their correlation with hepatoprotective effect.

Methods

The effects of Zhicaowu-Hezi formulation on CYP450 enzymes were systematically evaluated through integrated in vi

SUBMITTER: Zhu J 

PROVIDER: S-EPMC12301304 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature