Comparative diagnostic performance of metagenomic and two targeted sequencing methods in lower respiratory infection.

ABSTRACT: Lower respiratory tract infections are characterized by high morbidity and mortality, the latter associated with the low sensitivity and long turnaround time (TAT) of traditional diagnostic methods. Advances in next-generation sequencing (NGS) offers a promising solution, but in the face of so many different NGS products, how to use them appropriately remains a great challenge for clinicians. This study included 205 patients with suspected lower respiratory tract infections from the department of respiratory and critical care medicine, and collected their lower respiratory tract samples for metagenomic NGS (mNGS) and two different targeted NGS (tNGS), amplification-based tNGS and capture-based tNGS. We analyzed their microorganisms reported, and evaluated their detection performance based

SUBMITTER: Yi L 

PROVIDER: S-EPMC12301447 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature