14-3-3ε inhibits premature centriole disengagement by inhibiting the activity of Plk1 and separase.

ABSTRACT: The 14-3-3 protein family regulates several pathways in mammalian cells, including centrosome duplication. However, the precise mechanisms by which 14-3-3 paralogs regulate the centrosome cycle remain unclear. To identify the mechanisms by which 14-3-3ε regulates centrosome duplication, we altered two conserved acidic residues in the 14-3-3ε phospho-peptide-binding pocket that regulate complex formation and dissociation with the associated ligands, D127 and E134, to alanine. Altering these residues to alanine led to opposing effects on centrosome duplication; the D127A mutant inhibited centrosome duplication, whereas cells expressing the E134A mutant showed the presence of supernumerary centrosomes. We demonstrate that 14-3-3ε does not inhibit centriole duplication, as reported for 14-3-3γ

SUBMITTER: Jaiswal MA 

PROVIDER: S-EPMC12301659 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature