Design and characterisation of photoactivatable and lysine reactive <i>o</i>-nitrobenzyl alcohol-based crosslinkers.

ABSTRACT: Photoreactive groups are invaluable tools in structural proteomics, offering reagent-free activation and temporal control of protein labelling. However, traditional UV-activatable functional groups often produce unstable intermediates and diverse products, making these chemistries difficult to deploy at scale. In this study, we performed a systematic analysis of ortho-nitrobenzyl alcohol (oNBA) reactivity for integration into novel reagents for chemical crosslinking-mass spectrometry. oNBA photochemistry represents a promising alternative to traditional photoactivatable crosslinkers due to its unique specificity towards lysine residues. Here, we synthesised two molecules containing oNBA functional groups with different substituents and assessed their labelling e

SUBMITTER: Cahill A 

PROVIDER: S-EPMC12301895 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature