LIRTS Viewer: A Web-Based Resource to View the Transcriptional Response of Lens Epithelial Cells to Injury.

ABSTRACT:

Purpose

Residual lens epithelial cells (LECs) respond to injury after cataract surgery, leading to posterior capsular opacification (PCO). Transcriptomic profiling of lens capsule-associated cells (CACs) post-cataract surgery (PCS) revealed that LECs quickly alter their transcriptome, producing numerous pro-inflammatory cytokines within a few hours PCS. In contrast, the significant activation of TGFβ signaling and fibrotic extracellular matrix deposition related to PCO only begins 1 to 3 days later. However, the global changes in gene expression in CACs, following the establishment of robust TGFβ signaling, remain unknown.

Methods

Lens fiber cells were removed from wild-type mice, and CACs were isolated at 0, 72, or 120 hours PCS to perform bulk RNA sequencing (RNA-seq) to o

SUBMITTER: Gorai S 

PROVIDER: S-EPMC12302040 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature