Palmitate-Induced Primary Rat Senescent Astrocytes Exhibit Higher Inflammatory Activity and a Distinct Transcriptomic Profile Compared to Reactive Astrocytes.

ABSTRACT: Astrocytes play a crucial role in mediating neuroinflammation, particularly under pathological conditions where they can enter into senescent or gliotic states. This study explored the induction of these two astrocytic states using the same stressor, palmitate, in primary cortical astrocytes. A transcriptomic analysis revealed distinct expression profiles both astrocytes phenotypes. Senescent astrocytes upregulated genes involved in cell cycle arrest and the Senescence-Associated Secretory Phenotype (SASP), including IGFBP5, CDKN1A, and p53. In contrast, reactive astrocytes upregulated genes related to immune response, complement activation, and inflammation, such as C3, LCN2, and pro-inflammatory cytokines like IL-11 and CXCL12. Despite these differences, both astrocytic states shared pro

SUBMITTER: Lopez-Teros M 

PROVIDER: S-EPMC12302067 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature