High-throughput impedance monitoring in 3D tumor cultures: a multiplex, microfluidic-free platform for drug screening.

ABSTRACT: The development of an effective therapy against glioblastoma (GBM) remains a significant and unmet clinical need. To address this challenge, creating predictive, physiologically relevant screening models is essential for accelerating the identification of promising drug candidates. In this paper, we present a novel impedance-based device where two-photon polymerization-fabricated scaffolds embedding electrodes are colonized by GBM cells, effectively replicating the three-dimensional environment of the microscopic tumor foci that persist following tumor resection and cause recurrence. The results demonstrated that the proposed GBM-on-chip model enables high-throughput, multiplexed, and real-time monitoring of the development of tumor spheroids and their responses to therapeutic agents. Vali

SUBMITTER: Marino A 

PROVIDER: S-EPMC12302074 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature