The effects of primary cilia-mediated mechanical stimulation on nestin⁺-BMSCs during bone-tendon healing.

ABSTRACT:

Introduction

Mechanical stimulation has been proven to promote bone-tendon interface (BTI) healing, but the mechanism remains unclear.

Objective

To investigate the effects of mechanical stimulation on the biological behavior of nestin⁺-bone mesenchymal stem cells (BMSCs) during the BTI healing, and to reveal the mechanisms of mechanical stimulation affecting BTI healing by primary cilia on the nestin⁺-BMSCs.

Methods

Transgenic tracing mice (nestin cre^ERT2:: IFT88^fl/fl/ROSA26 YFP) with primary cilia on nestin⁺-BMSCs conditioned knocked out were constructed, and the littermates (nestin cre^ERT2:: ROSA26 YFP) with normal cilia on nestin⁺-BMSCs were the control. After establishing mouse supraspina

SUBMITTER: Chen H 

PROVIDER: S-EPMC12302343 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature