Numbers of Exchangeable Hydrogens from LC-MS Data of Heavy Water Metabolically Labeled Samples.

ABSTRACT: Labeling with deuterium oxide (D₂O) has emerged as one of the preferred approaches for measuring the synthesis of individual proteins in vivo. In these experiments, the synthesis rates of proteins are determined by modeling mass shifts in peptides during the labeling period. This modeling depends on a theoretical maximum enrichment determined by the number of labeling sites (N_EH) of each amino acid in the peptide sequence. Currently, N_EH is determined from one set of published values. However, it has been demonstrated that N_EH can differ between species and potentially tissues. The goal of this work was to determine the number of N_EH for each amino acid within a given experiment to capture the conditions uni

SUBMITTER: Deberneh HM 

PROVIDER: S-EPMC12302360 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature