Vasoactive intestinal peptide reduces ocular hypertension by regulating tight junction of trabecular meshwork through Rab13/PKA signalling complex.

ABSTRACT: Schlemm's canal and trabecular meshwork (TM) are conventional outflow pathway of aqueous humour, which play an important role in maintaining intraocular pressure (IOP) homeostasis. Our previous research has implied that vasoactive intestinal peptide (VIP) reduces high IOP by regulating the distribution of F-actin in the endothelial cells of Schlemm's canal. In this study, we investigated the mechanism by which VIP affects tight junction (TJ) of TM. Burning three episcleral veins in the eye of rats and pressure-stimulating human trabecular meshwork cells (HTMCs) were respectively employed to establish high IOP animal and cell models. The IOP of the rats was measured by TonoLab during the period of 1-2 min after isoflurane anaesthesia at 10:00 to 11:00 a.m. on the measurement day. The tissue

SUBMITTER: Chen L 

PROVIDER: S-EPMC12302395 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature