Optimizing therapeutic efficacy of antifungal peptides via strategic terminal amino acid modification.

ABSTRACT:

Introduction

Antifungal peptides (AFPs) have the potential to treat antifungal-resistant infections; however, their structure-function relationship remains unknown, hindering their rapid development. Therefore, it is imperative to investigate and clarify the structure-function relationships of AFPs.

Objectives

This study aimed to investigate the impact of end-tagging single hydrophobic amino acids and capping the N-terminus with glycine (Gly) on the antifungal activity of peptide W4.

Methods

The antifungal efficacy of the engineered peptides was initially assessed by determining the minimum inhibitory concentration (MIC) /minimal fungicidal concentration (MFC), killing kinetics, and drug resistance induction, in addition to evaluating the biocompatibility and stabilit

SUBMITTER: Song J 

PROVIDER: S-EPMC12302477 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature