RBOHD, GLR3.3, and GLR3.6 cooperatively control wounding hypocotyl-induced systemic Ca²⁺ signals, jasmonic acid, and glucosinolates in Arabidopsis leaves.

ABSTRACT: Ca²⁺ signaling plays crucial roles in plant stress responses, including defense against insects. To counteract insect feeding, different parts of a plant deploy systemic signaling to communicate and coordinate defense responses, but little is known about the underlying mechanisms. In this study, micrografting, in vivo imaging of Ca²⁺ and reactive oxygen species (ROS), quantification of jasmonic acid (JA) and defensive metabolites, and bioassay were used to study how Arabidopsis seedlings regulate systemic responses in leaves after hypocotyls are wounded. We show that wounding hypocotyls rapidly activated both Ca²⁺ and ROS signals in leaves. RBOHD, which functions to produce ROS, along with two glutamate receptors GLR3.3 and GLR3.6, but not individually RBOH

SUBMITTER: Zhan C 

PROVIDER: S-EPMC12302501 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature