Development of a DNA Aptamer-Based Approach to Noninvasively Image CAR-T Cells In Vivo and Traceless Enrichment In Vitro.

ABSTRACT: Chimeric antigen receptor (CAR) T cells offered a potential cure for malignancies, however, their outcomes and dynamics across different anatomical sites remained inadequately characterized. Monitoring the bio-distribution and tumor-homing of CAR-T cells in vivo is crucial, as it provides patient-specific data that might inform on treatment success, potential failure, and off-target toxicities. Herein, an Aptamer A3 by Cell-SELEX (systematic evolution of ligands by exponential enrichment) is generated, which can bind with CAR-T cells with nanomolar affinity. After CAR-T cells are injected into Nalm6 xenograft tumor model mice through tail vein, Cy5-labeled A3 is injected into mice for fluorescence time-delay imaging in vivo. The fluorescence signal produced by the Cy5-labeled A3 is accumul

SUBMITTER: Chen M 

PROVIDER: S-EPMC12302554 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature