Mechanical Stress Induced NOX2 Promotes Endothelial Dysfunction in Ventilator-Induced Lung Injury: Potential Treatment with Quercetin.

ABSTRACT: Mechanical ventilation (MV) is a treatment that helps people who are unable to breathe on their own. However, the use of MV leads to the development of ventilator-induced lung injury (VILI). Here, it is found that VILI with endothelial barrier disruption is accompanied by elevated reactive oxygen species (ROS) in mice. NADPH oxidase 2 (NOX2, also known as CYBB or gp91phox) is first examined to be the main source of ROS to repress endothelial junction. Besides, 20%-0.5 Hz high cyclic stretch (high CS) significantly increases NOX2 expression and inhibits endothelial junction protein expression. NOX2 activates the downstream Calcium-calmodulin (CaM)-dependent protein kinase II (CaMKII)/ Extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathway through the regulation of ROS.

SUBMITTER: Jiang T 

PROVIDER: S-EPMC12302586 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature