CtDNA methylation profiling reveals NBL1 as a promising biomarker for early ovarian cancer screening.

ABSTRACT:

Background

Early detection of epithelial ovarian cancer (EOC) is crucial for improving patient survival. Current screening methods have limitations, highlighting the need for novel biomarkers. Circulating tumor DNA (ctDNA) methylation analysis offers a promising approach.

Methods

This study included 10 patients with EOC and 10 patients with benign pelvic masses. We collected plasma samples from these patients and isolated ctDNA. We then conducted whole-genome methylation sequencing using the TAPS (TET-assisted pyridine borane sequencing) method, which allows for single-base resolution detection of 5-methylcytosine and 5-hydroxymethylcytosine. Bioinformatics analysis was performed to identify differentially methylated genes and regions. We further validated candidate biomarke

SUBMITTER: Zhou S 

PROVIDER: S-EPMC12302688 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature