NSUN2 inhibits NCOA4 expression to alleviate ferroptosis and inflammation in sepsis-induced myocardial injury in a m⁵C manner.

ABSTRACT:

Background

Sepsis-induced myocardial injury (SIMI) leads to high morbidity and mortality. The 5-methylcytosine (m⁵C) RNA methyltransferase NOL1/NOP2/SUN domain (NSUN)2 is a therapeutic target for many diseases. The purpose of this study was to investigate the role of NSUN2 in SIMI and the potential mechanism.

Methods

Both an in vivo cecum ligation and puncture (CLP) SIMI rat model and an in vitro lipopolysaccharide (LPS)-treated H9c2 cardiomyocytes model were established. Reverse transcription (RT)-quantitative polymerase chain reaction (qPCR) was used to detect m⁵C-related and ferroptosis-related mRNA levels. Enzyme-linked immunosorbent assay was performed to assess inflammatory cytokines levels. Ferroptosis-related indicators were detected by commerci

SUBMITTER: BoRanyi B 

PROVIDER: S-EPMC12302838 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature