DODAB-ODN lipoplex structure is highly dependent on ODN concentration: A multitechnique experimental study.

ABSTRACT: This study explores the structural properties of lipid vesicles composed of dioctadecyldimethylammonium bromide (DODAB) and varying concentrations of the oligonucleotide 5'-AAAAAAAAAA-3' (ODN). The vesicles, in both gel and fluid phases, were characterized using dynamic light scattering (DLS), Zeta potential, small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and fluorescence with the fluorescent probe Laurdan embedded into DODAB vesicles. The DODAB-ODN interaction induces concentration-dependent structural changes on the lipoplex. At relatively low ODN concentrations ([ODN]/[DODAB] < 0.050), the dispersion remains stable, with positively charged vesicles, though with an increase in vesicle size and sample turbidity, and SAXS indicates coalescence of vesicles wit

SUBMITTER: Vivas CV 

PROVIDER: S-EPMC12302854 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature