Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers.

ABSTRACT:

Background

The discovery of CRISPR/Cas9 and its subsequent accessibility in daily research initiated a new era in genome editing. This game-changing genetic instrument enabled a vast array of challenging applications requiring site-specific genome engineering as well as applications involving the equipment of cells with additional genetic traits. Despite the undisputed benefits of this technology, for facile and efficient selection of successfully manipulated cells selectable markers remain indispensable. Over the past years endogenous counter-selectable markers have come into focus in antifungal research enabling site-directed integration of multiple genes into the genome of the human mold pathogen Aspergillus fumigatus. However, gene cassettes had to be transformed in a consecuti

SUBMITTER: Sastre-Velasquez LE 

PROVIDER: S-EPMC12302872 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature