Collagen binding properties separate two functionally distinct subpopulations of milk extracellular vesicles regarding bone regenerative capacity.

ABSTRACT: Extracellular vesicles (EVs) are heterogeneous in their composition. The proteins on their surface determine their binding properties to the meshwork of extracellular matrix (ECM). Here, we report that type I collagen-binding property separates two subpopulations of EVs from cow milk (mEVs): collagen-binding mEVs (^{cb +}mEVs) and non-collagen binding mEVs (^cb-mEVs). ^{cb +}mEVs showed noticeable uptake by human bone marrow mesenchymal stromal cells (hBMSCs) and osteogenic functionality in vitro (1.2-fold increase in mineralization). By proteomics profiling we identified Annexin V (AnxV) and confirmed its enrichment on CD9 positive ^{cb +}mEVs using immunomagnetic separation. By implanting a hydrogel construct enric

SUBMITTER: Wang P 

PROVIDER: S-EPMC12302926 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature