ASXL2 Modulates Chromatin Remodeling to Direct Osteogenesis and Multiple Cell Fate in hPDLSCs.

ABSTRACT:

Aims

This study investigates the epigenetic role of Additional Sex Combs-Like 2 (ASXL2) in regulating osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs), aiming to address limitations in current strategies for oral and maxillofacial tissue regeneration.

Methods

Lentiviral-mediated ASXL2 knockdown in hPDLSCs was validated by flow cytometry. Functional impacts were assessed through proliferation/apoptosis assays, alkaline phosphatase (ALP) activity, Alizarin Red mineralization, and qPCR/Western blot analyses of osteogenic markers (RUNX2, ALP, COL1A1, OCN). Global histone modification dynamics (H2AK119ub, H3K27me3, H3K4me3) were detected to elucidate epigenetic mechanisms.

Results

ASXL2 depletion enhanced hPDLSC proliferation and reduced apopto

SUBMITTER: Yang T 

PROVIDER: S-EPMC12303053 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature