Discovery of Cryptic Natural Products Using High-Throughput Elicitor Screening on Agar Media.

ABSTRACT: It is now well-established that microbial genomes carry sparingly expressed biosynthetic gene clusters (BGCs) that need to be induced in order to characterize their products. To do so, we herein subjected two well-known producers, Burkholderia plantarii and Burkholderia gladioli, to high-throughput elicitor screening (HiTES), an emerging approach for accessing the products of these "cryptic" BGCs. Both organisms have previously been examined extensively in liquid cultures. We therefore applied HiTES on agar and found several novel natural products that are only produced in this format and not in liquid cultures. Most notably we found two metabolites, termed burkethyl A and B, that contain an unusual m-ethylbenzoyl group and for which we identified the cognate BGC using

SUBMITTER: Lee SR 

PROVIDER: S-EPMC12303134 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature