Vitamin D and lipopolysaccharide jointly induce a distinct epigenetic and transcriptional program in human monocytes.

ABSTRACT: Pathogen-associated molecular patterns such as lipopolysaccharide (LPS) mimic immune responses triggered by bacterial infections. The hormonally active form of vitamin D₃, 1α,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃], supports innate immunity, but its molecular mechanisms remain incompletely understood. We investigated epigenomic and transcriptomic changes in THP-1 monocytes that were either unprimed or primed for 24 h with 1,25(OH)₂D₃ or LPS, followed by a second 24-hour stimulation with 1,25(OH)₂D₃, LPS, or their combination. Epigenome profiling via ATAC-seq revealed that co-stimulation with 1,25(OH)₂D₃ and LPS induces substantially more chromatin accessibility changes than either tre

SUBMITTER: Jankowski M 

PROVIDER: S-EPMC12304155 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature