SARM1 Inhibition Maintains Axonal Integrity After Rat Sciatic Nerve Transection and Repair.

ABSTRACT:

Purpose

Sterile alpha and TlR motif containing-1 (SARM1) protein has been demonstrated to play a critical role in the initiation of Wallerian degeneration after nerve injury. The goal of this study was to assess whether blockade of SARM1 activity inhibits Wallerian degeneration following nerve transection, potentially promoting more rapid recovery of axonal function.

Methods

An adeno-associated virus plasmid encoded with a dominant-negative SARM1 protein fused with green fluorescent protein to impair SARM1 function, was injected into 24 juvenile rats to create a SARM1 dominant-negative (SARM1-DN) phenotype. Twenty-four control rats were injected with a control plasmid expressing only green fluorescent protein. Three weeks after transfection, the rats underwent unilateral sci

SUBMITTER: Sachar R 

PROVIDER: S-EPMC12304245 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature