Engineering of photo-inducible binary interaction tools for biomedical applications.

ABSTRACT: The ssrA-sspB dimerization system, derived from the bacterial degradation machinery, comprises a 7-residue ssrA peptide and its binding partner sspB. The compact size of ssrA makes it ideal for insertion into proteins of interest to manipulate host protein function by engineered light-responsive sspB. In contrast to the LOV2 caging strategy employed to develop optical dimerizers, we present herein two distinct photo-inducible binary interaction tools (PhoBITs) systems: PhoBIT1, a light-OFF switch generated by integrating LOV2 into sspB, and PhoBIT2, a light-ON switch building upon an evolved ssrA/CRY2-sspB pair with minimal basal interaction. These tools enable mechanistic dissection and optogenetic modulation of GPCRs, ion channels, necroptosis, and innate immune signaling. When incorpora

SUBMITTER: Lee YT 

PROVIDER: S-EPMC12304284 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature