Platelet-Activating Factor Promotes Neutrophil Activation and Platelet-Neutrophil Complex Formation.

ABSTRACT: Controlling excessive inflammation remains an unmet clinical need, for example, during sepsis or after severe injuries. Platelet-activating factor (PAF) is central in thromboinflammatory processes. However, its role in the interaction of platelets and neutrophils requires further insights. Therefore, we elucidated PAF-related neutrophil activation, including platelet-neutrophil complex (PNC) formation and investigated potential strategies to modulate PAF-related inflammation. For the translation of the PAF-mediated inflammation, we applied an animal-free human ex vivo whole blood model. The neutrophil phenotype, its function, and PNC formation were studied by flow cytometry and platelet-related activity was assessed by light microscopy and aggregometry. PAF induced a rapid and dose-depende

SUBMITTER: Wohlgemuth L 

PROVIDER: S-EPMC12304291 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature