Knockdown of CSF1R molecules enhances the antitumor effects of CD8⁺ T lymphocytes in bladder cancer.

ABSTRACT:

Objective

In this study, we preliminarily investigated the efffects of the colony stimulating factor 1 receptor (CSF1R) molecules on CD8⁺ T cells in bladder cancer (BLCA) and the underlying molecular mechanism.

Methods

The effects of CSF1R in CD8⁺ T cells on BLCA cell proliferation, migration and, invasion were determined by cell-killing assay and transwell assays.For in vivo experiments, tumours tissues were divided into four portions: (1) histological staining, (2) flow cytometry detection, (3) mRNA gene sequencing analysis, and (4) qPCR validation. Information related to 161 bladder cancer patients with BLCA at Xiangya Hospital of Central South University from 2019 to 2023 was collected. The pathological tissues were subjected to immunofluorescence.

SUBMITTER: Peng J 

PROVIDER: S-EPMC12304351 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature