Label-Free Detection of Virus-Membrane Interactions Using Surface-Enhanced Infrared Absorption (SEIRA) Spectroscopy.

ABSTRACT: Viral binding and membrane fusion are essential steps in viral infection, mediated by viral proteins that bind to host cell receptors and facilitate the fusion between viral and host membranes. Targeting these steps for the development of new antiviral strategies requires methods that enable investigating virus-membrane interactions under in-situ conditions, while providing mechanistic insights on a molecular level. Here, we demonstrate the use of surface-enhanced infrared absorption (SEIRA) spectroscopy combined with tethered bilayer lipid membranes (tBLMs) for the label-free detection of virus-membrane interactions, using the Influenza A/X-31 virus (IAV) as a model. Exploiting the nanometer-scale surface-sensitivity of SEIRA, we detect the vibrational fingerprint of IAV's hemagglutinin (

SUBMITTER: Heinen AT 

PROVIDER: S-EPMC12304793 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature