Implementing BMP-7 Chemically Modified RNA for Bone Regeneration with 3D Printable Hyaluronic Acid-Collagen Granular Gels.

ABSTRACT: Chemically modified RNA (cmRNA) is emerging as a more effective alternative to protein delivery and DNA-based gene therapy. To implement this technology for bone regeneration, a suitable biomaterial functioning as scaffold and delivery system is necessary. This study introduces a 3D printable granular hydrogel consisting of hyaluronic acid and collagen (THA-Col) for the delivery of bone morphogenetic protein (BMP)-7 cmRNA as activated matrix to promote bone healing. Granular hydrogels are produced by mechanically fragmenting bulk THA-Col gels. Resulting microgels are 3D printable and are further investigated in comparison to bulk THA-Col gels for BMP-7 cmRNA transfection efficiency, cytotoxicity, and osteogenic differentiation of human mesenchymal stromal cells (hMSCs). Microgels showed hi

SUBMITTER: van der Heide D 

PROVIDER: S-EPMC12304813 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature