Effect of Nanoparticle Rigidity on the Interaction of Stromal Membrane Particles with Leukemia Cells.

ABSTRACT: In acute myeloid leukemia (AML), disease relapse is often observed because of therapy-resistant leukemic stem cells that re-initiate the disease. Leukemic stem cells can tightly associate with mesenchymal stromal cells inside the bone marrow, which is considered to further drive drug resistance. Here, the cell membrane of bone marrow stromal cells is used to prepare cell membrane nanoparticles and study their interactions with AML cells. Cell membrane liposomes (CM-Liposomes) of different charge are prepared and either used directly, or after deposition on silica cores to modulate nanoparticle mechanical properties. Nanoparticle size, zeta potential and coating efficiency are analyzed by dynamic light scattering (DLS) and cryo electron microscopy (Cryo-EM) imaging. Atomic force microscopy

SUBMITTER: de Weerd S 

PROVIDER: S-EPMC12304856 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature