Pannexin-1 channels, extracellular ATP, and purinergic receptors are essential for CCR5/CXCR4 clustering and HIV entry.

ABSTRACT:

Objective

The Human Immunodeficiency Virus-1 (HIV) cell entry has been well characterized with the identification of CD4 as the main receptor and CXCR4 and CCR5 as co-receptors for the virus. However, how the virus uses the cell machinery for entry and infection is still a work-in-progress. Previously, we identified that the Pannexin-1 (Panx-1) channel, extracellular ATP, and purinergic receptors axis are essential for HIV entry and replication in macrophages, but the mechanisms were not fully explored.

Methods

Electrophysiology, ATP quantifications, confocal, HIV entry and replication experiments were used to determine the role of Panx-1 channels in HIV entry.

Results

Here, we identified that HIV or gp120 induces Panx-1 channel opening in association with ATP secreti

SUBMITTER: Ajasin D 

PROVIDER: S-EPMC12304881 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature