An enzyme-based approach for highly efficient self-replication of DNA origami dimers.

ABSTRACT: Self-replication and exponential growth are essential to all living things, the driving force for Darwinian evolution, and potentially useful in nanotechnology for large-scale production of nanoscopic materials. An artificial (nonliving) self-replication system has been shown to exhibit exponential growth and selection using DNA monomer origami tiles templated on a dimer seed. That system purposefully avoided the use of enzymes to get a hint of how self-replication might have evolved in a prebiotic world by using ^CNVK and UV light to crosslink complementary DNA single strands. For further investigations into competition and extinction and for potential applications involving biocompatibility, we wanted to investigate enzymatic ligation to replace the chemical photo crosslinking

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12304977 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature