Protocol for conducting a single-cell sequencing assay for transposase-accessible chromatin analysis.

ABSTRACT: While the single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) is a powerful single-cell resolution tool for studying chromatin accessibility, its analytical workflow presents significant challenges for researchers new to scATAC-seq. Here, we present a protocol for conducting scATAC-seq analysis using a publicly available dataset as an example. We describe steps for data pre-processing and downstream analysis. We then detail procedures for computational multi-omics integration.

SUBMITTER: Ma W 

PROVIDER: S-EPMC12305206 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature