Native Top-Down Analysis of Membrane Protein Complexes Directly From In Vitro and Native Membranes.

ABSTRACT: Macromolecular organization of proteins and lipids in cellular membranes is fundamental to cell functionality. Recent advances in native mass spectrometry (nMS) have established it as a key analytical tool for capturing these associations. This typically necessitates the extraction of target membrane proteins (MPs) from their physiological environments into detergent-like surroundings. In our recent studies using in vitro synthetic liposomes, we discovered that gas phase supercharging can selectively destabilize lipid bilayers and enable MS1 detection of embedded and associated protein-lipid complexes. Here, we further extend and apply this methodology to native cell-derived membrane vesicles. We demonstrate our ability to detect and ID protein complexes and their proteoforms directly from

SUBMITTER: Jung W 

PROVIDER: S-EPMC12305242 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature