Transcriptomic insights into host transcriptional manipulation by ssDNA and ssRNA viruses in the marine planktonic diatom Chaetoceros tenuissimus.

ABSTRACT: The marine diatom Chaetoceros tenuissimus coexists in the coastal ocean with two distinct viruses: a circular single-stranded DNA virus and a single-stranded RNA virus. Both viruses are capable of killing the host in vitro experiments; however, the molecular mechanisms by which host genes respond and are utilized by these viruses for replication and proliferation remain unclear. In this study, RNA-seq analyses were conducted to investigate these mechanisms, and the host transcripts specific to each virus were validated by RT-qPCR. In the early phase of infection, RNA-seq analysis revealed that gene transcription related to endocytosis, vacuolar acidification, and nitrogen metabolism was commonly upregulated. In contrast, genes related to transcription and translation were downregulated in

SUBMITTER: Hongo Y 

PROVIDER: S-EPMC12305248 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature