The three-dimensional structure prediction of human bitter taste receptor using the method of AlphaFold3.

ABSTRACT: Bitter taste receptors (T2Rs), a subfamily of G protein-coupled receptors, are expressed not only in oral tissues but also in extraoral sites, playing key roles in physiological processes such as the gut-brain axis. However, structural information on T2Rs is limited, with only two human T2Rs, T2R14 and T2R46, experimentally determined to date. This study explores the potential of AlphaFold3 (AF3), an advanced AI-based protein structure prediction tool, to predict the structures of 25 human T2Rs and compares them with those of the earlier AlphaFold2 (AF2). The accuracy of AF3 was evaluated by comparing the predicted structures of T2R14 and T2R46 with known experimental structures. Our results show that AF3 provides more accurate structural predictions than AF2 for these receptors, though th

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC12305321 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature