Intestinal KLHL12 is dispensable for lipid absorption and chylomicron metabolism.

ABSTRACT: Kelch-like protein 12 (KLHL12) has been shown to regulate coat complex II (COPII)-mediated endoplasmic reticulum (ER)-to-Golgi trafficking of large cargos carrying procollagen or apolipoprotein B-100 containing very-low-density lipoprotein (VLDL). It is known that lipid absorption and chylomicron metabolism in enterocytes are dependent on apolipoprotein B-48 (ApoB48) and COPII-mediated trafficking. This study aimed to investigate whether KLHL12 in the intestine regulates dietary lipid absorption, chylomicron assembly, and metabolic phenotypes in mice. We generated Klhl12 intestinal-specific knockout (IKO) mice and assessed the impact of its deficiency on lipid absorption and Western diet (WD)-induced obesity in both male and female mice. We examined lipid absorption in vivo by acute

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC12305500 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature